Beta-actin is one of the six identified isoforms of actin. Beta-actin is a non-muscle cytoskeletal protein found in all types of human cells and is involved in cell motility, structure and integrity. There are six different isoforms of actin in humans. The beta-actin isoform coexists with gamma actin in most cell types as a component of the cytoskeleton.
Beta-actin is a cytoplasmic protein that is ubiquitously expressed in all eukaryotic cells. When globular actin (G-actin) polymerizes, a structural strand (F-actin) is formed in the form of a double-stranded helix. Actin is a highly conserved protein involved in cellular motility, structure and integrity. You can also have a peek here to know more about actin antibodies.
Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is often used as a loading control for assays involving protein detection, such as B. Western blotting. Antibodies to beta-actin provide a specific and useful tool for studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Use anti-beta-actin to detect beta-actin by ELISA, immunohistology, western blotting, and immunofluorescence. Antibodies specifically recognize beta-actin in various tissues and species using immunoblotting, immunofluorescence staining of cultured cell lines, and immunohistochemistry. Epitopes recognized by antibodies are resistant to formalin fixation and paraffin incorporation.
The anti-beta-actin monoclonal antibody can be used to analyze beta-actin protein expression in experimental cells by immunofluorescence and western blotting, and specifically used in combination with control beta-actin siRNA to determine the specificity and potential of RNA interference (RNAi) action at the level of proteins.