Western blotting is a straightforward but effective method for determining the relative abundance, presence, relative mass, and existence of post-translational modifications as well as protein-protein interactions. You can look for the best western blot analyzing service via bosterbio.
Image Source: Google
These Western blot applications are useful in academic research, as well as diagnostic and therapeutic testing. After the target protein has been isolated on a protein gel, secondary and primary antibodies are used to bind and visualize it.
The Western blot was designed to give a yes/no answer to whether a target protein was present in a protein sample. This qualitative method uses a simple visual assessment to establish the presence of target bands.
The scientific community is concerned about Western blot analysis and other immunoassays. The immunoblotting procedure entails a complex set of interconnected phases that are influenced by user preferences and expertise. Experiment design, approach, and procedure variations can all be significant sources of mistakes.
This unpredictability is especially concerning when it comes to quantitative analysis of Western blot data when an inaccuracy could result in data misinterpretation.
Differences in the reagents and parameters employed for each experiment that appear to be tiny or inconsequential can have a surprising impact on the results. To avoid frequent mistakes and determine which assay limitations are most relevant for reproducible results, careful experimental design and well-characterized procedures are required.